Wednesday, October 30, 2019

The Evaluation of the Speed-Oligo® Mycobacteria Assay for Identification of Mycobacterium spp. from Smear Positive and Negative Sputum Samples: Crimson Publishers

The Evaluation of the Speed-Oligo® Mycobacteria Assay for Identification of Mycobacterium spp. from Smear Positive and Negative Sputum Samples by Gülnur Tarhan in Cohesive Journal of Microbiology & Infectious Disease



Speed-oligo® Mycobacteria is an oligochromatographic test for the qualitative detection of Mycobacterium genus and the species of M. tuberculosis complex, directly in clinical samples. It is based on polymerase chain reaction targeting 16S rRNA and 16S-23S rRNA regions and double-reverse hybridization on a dipstick using probes bound to colloidal gold and to the membrane. In this study, we evaluated 25 type reference strains of mycobacteria (18 non-tuberculosis mycobacteria, M. tuberculosis H37Rv and M. tuberculosis H37Ra), 60 sputum samples (40 smear positive, 20 smear negative) collected from patient with suspected TB. All results were compared with microscopy, Löwenstein Jensen culture and Inno-Lipa (GenoType Mycobacterium CM/AS; Hain Lifescience, Germany). All smear positive sputum samples were positive with microscopy, culture and Speed-oligo® Mycobacteria. Of 20 smear negative sputum samples, 7 were culture positive. Of 7 culture positive samples, 3 were positive with microscopy and Speedoligo ® Mycobacteria. It is not effective to identify for M. intermedium, M. kansasii and M. xenopi.

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Tuesday, October 22, 2019

Molecular Dıagnostic Tests Used in the Dıagnosis of Tuberculosis:Crimson Publishers

Molecular Dıagnostic Tests Used in the Dıagnosis of Tuberculosis: Crimson publishers_Cohesive Journal of Microbiology &Infectious Disease:  Infectious DiseaseOpen Access Journal: Crimson Publishers

The definitive diagnosis of tuberculosis (TB) is dependent on the isolation, identification and drug susceptibility testing of the causal agent Mycobacterium tuberculosis by cultivation. Smear microscopy has poor sensitivity and culture is slow to yield results. The resurgence of tuberculosis worldwide has been accompanied by an increase in the incidence of multidrug-resistant (MDR) tuberculosis on all continents. At the same time, a number of other nontuberculous mycobacterial (NTM) species are emerging as causes of disease. A quick and correct diagnosis of symptomatic tuberculosis is critical for the control of this serious disease. The nucleic acid amplification techniques (NAATs) and other molecular biology methods (i .e. DNA hybridization, DNA sequencing, etc.) are essential in today’s laboratory practices for detection and characterization of mycobacteria.


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Sunday, September 29, 2019

The Place Importance of Serologic Techniques in Tuberculosis Dıagnosis_Crimson Publishers


The Place Importance of Serologic Techniques in Tuberculosis Dıagnosis by Gülnur Tarhan in Cohesive Journal of Microbiology & InfectiousDisease: Crimson Publishers_Infectious Disease Open Access Journals

Diagnosing active TB accurately and rapidly is a key challenge for eradicating the TB epidemic. Conventional culture methods are slow and staining methods not sufficiently sensitive. Nucleic acid amplification techniques (NAATs) tend to be costly and in some cases lack sensitivity. Antibody detection tests (serological tests) have a long history and have been used successfully for the rapid diagnosis of many infectious diseases (e.g., HIV, syphilis, and viral hepatitis). TB serological tests almost exclusively rely on antibody recognition of antigens of Mycobacterium tuberculosis by the humoral immune response, as opposed to antigen recognition by the cellular immune response (e.g. interferon-gamma release assays. These tests use various modifications of enzyme-linked immunosorbent assay (ELISA) or immunochromatographic methods to detect different antibody classes. Cellular immunodiagnostics, including tuberculin skin test (TST) and Interferon-Gamma Release Assays (IGRAs) have been used to diagnose latent tuberculosis infection (LTBI). The TST is the only universally accepted test for the diagnosis of LTBI.


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Wednesday, September 25, 2019

Sunday, September 15, 2019

Molecular Study of Cutaneous Leishmaniasis Human Reservoirs and Infections in Bastak_Crimson Publishers

Molecular Study of Cutaneous Leishmaniasis Human Reservoirs and Infections in Bastak: Crimson Publishers: Cohesive Journal of microbiology & infectious disease_Journal of Microbiology




Leishmaniasis is one the six most important diseases in tropical areas. Study and research on various aspects of it is recommended and supported by the World Health Organization. Broom tail rodents (Rodentia: Muridae: Gerbillinae) are the most important reservoir host of parasite causes wet cutaneous leishmaniasis (or Zoonotic Cutaneous Leishmaniasis (ZCL) disease) means leishmania major. Different species of rodents from different regions of Iran play role in maintenance of the parasite. The purpose of this research is molecular study of cutaneous leishmaniasis human reservoirs and infections in Bastak County, Hormozgan Province, Iran



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Monday, September 9, 2019

The Magnetic Field Therapies in Association with the Relation between Macro and Microcosmos:_ Crimson Publishers

The Magnetic Field Therapies in Association with the Relation between Macro and Microcosmos: Cohesive Journal of Microbiology & Infectious Disease_Crimson Publishers: Journal of Microbiology



From the concept of energy and matter to the magnetic fields in the universe, the relation between macro and micro points of view gives new perspectives for the understanding and management of life. While the discoveries of huge magnetic fields and mapping those are helping about galaxy and space formation [1,2], the magnetic fields of human body give information about the situation of cells, organs and the hole organism [3].
Kirlian photos and aura visualization give the first attempts of structuring electromagnetic fields of human being [4]. In addition to the match between the seven chakras in human body, the Solfeggio frequencies and visible light spectrum, it has shown that the solar activity as Schumann resonance sychronized with the frequency of organisms affects especially the human health [5].
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Friday, August 30, 2019

Crimson Publishers: The Usage of a Locomotive Tractive Power in the Di...

Crimson Publishers: The Usage of a Locomotive Tractive Power in the Di...: The Usage of a Locomotive Tractive Power in the Different Operating Modes and the Efficiency Control by Alexander Jr Zarifyan in Crimson ...

Crimson Publishers: Nanocellulose in Food Science and Technology - Pot...

Crimson Publishers: Nanocellulose in Food Science and Technology - Pot...: Nanocellulose in Food Science and Technology - Potential, Advantages and Gaps of Research by Talita Szlapak Franco in Food science  ...

Crimson Publishers: C-Arm Free Technique for Anterior Correction of Ad...

Crimson Publishers: C-Arm Free Technique for Anterior Correction of Ad...: C-Arm Free Technique for Anterior Correction of Adolescent Idiopathic Scoliosis: A Technical Note by Tanaka M* in Researches in Arthriti...

Crimson Publishers: C-Arm Free Technique for Anterior Correction of Ad...

Crimson Publishers: C-Arm Free Technique for Anterior Correction of Ad...: C-Arm Free Technique for Anterior Correction of Adolescent Idiopathic Scoliosis: A Technical Note by Tanaka M* in Researches in Arthriti...

Friday, August 23, 2019

Viable Monitoring During the Filling of a Terminally Sterilized Pharmaceutical Product-Risk Based Approach-Crimson Publishers

Viable Monitoring During the Filling of a Terminally Sterilized Pharmaceutical Product-Risk Based Approach by Ahmed Assem in Infectious Disease Open Access Journals_Crimson Publishers

This article addresses a risk based approach to establish a routine monitoring program of viable (I.e. microorganisms) in a controlled environment used to produce pharmaceutical products that are intended to be terminally sterilized. Current applicable quality system regulation & GMP require appropriate environment to be established, maintained and monitored for the manufacturing of the terminally sterilized pharmaceutical meanwhile the appropriate environmental monitoring plan is not clearly specified & a risk based approach will be a valuable tool to design a suitable program

https://crimsonpublishers.com/cjmi/fulltext/CJMI.000527.php


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Friday, June 21, 2019

Huge Genetic Inclusion during Conversion of R-Plasmids into MDR Conjugative Plasmids in Multidrug-Resistant Bacteria: Crimson Publishers

Huge Genetic Inclusion during Conversion of R-Plasmids into MDR Conjugative Plasmids in Multidrug-Resistant Bacteria by Asit Kumar Chakraborty in Cohesive Journal of Microbiology & Infectious Disease: Crimson Publishers_Open Access High Impact Articles



High rate infections and deaths due to multidrug-resistant pathogens have created a horror worldwide. Because MDR bacteria are present in river, sea and air as well as in all household matters, such infections are ubiquitous. Most dangerous step occurred when R-plasmids and integrons (2-9kb) were combined with F’-conjugative plasmid (50-500kb) creating MDR conjugative plasmids that highly donated the mdr genes into household pathogens in the environment. Now 40% of household Escherichia coli and Staphylococcus aureus like Gram (+) and Gram (-) bacteria are ampicillin resistant and also to some extent tetracycline, chloramphenicol, streptomycin, erythromycin, ciprofloxacin, sulfamethoxazole and neomycin resistant.



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Friday, June 7, 2019

Prevalence of Coccidiosis in Back Yard Chicken in and Around DebereTabere Town, South Gondar Zone, Amhara Regional State, Ethiopia: Crimson Publishers

Prevalence of Coccidiosis in Back Yard Chicken in and Around DebereTabere Town, South Gondar Zone, Amhara Regional State, Ethiopia by Temesigen W Molla in Cohesive Journal of Microbiology & Infectious Disease: Crimson Publishers_World journal of microbiology and biotechnology

A cross sectional study was carried out to estimate the prevalence of chicken coccidial infections and assessment of the associated risk factor in backyard chickens in and around Debre Tabore town, South Gondar Zone, Northwest Ethiopia from October 2015 and May 2016.Floatation techniques were used for qualitative study of coccidialoocyst. Descriptive statistics was used to express prevalence while chi-square test used to assess if there was statistically significant association between targeted host factors and coccidial infection. The degrees of these associations were quantified using crude and adjusted odds ratio. Statistical significance was assumed if the confidence interval (CI) did not include one among its values or whenever p-value was less than 5%. The overall animal prevalence for coccidial infections was 21.4% (48/224) and the prevalence was significantly associated with breed (p=0.019), age (p=0.028) and sex (p=0.004) of the study animals. In a multivariable logistic regression analysis, female (Adjusted Odds Ratio (AOR)=0.3; 95%CI:0.17-0.68) and exotic breeds (AOR=0.46; 95%CI:0.23-0.93) were found less likely infected with coccidialoocystes as compared to male and local chickens. However, increased risk for coccidial infection was recorded in adult chicken (AOR=2.04; 95%CI:1.02-4.08) as compared to the grower chickens. In conclusion, this study showed coccidial infection could be important in the backyard chickens in the study area even though the overall prevalence was relatively low. Moreover, the prevalence was higher in adults than the growers, which alerts the need to undertake suitable and practically applicable control and prevention measures in the parent stocks.
 
 
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Saturday, May 25, 2019

AN EVALUATION ON ORAL VESTIBULAR SCHWANNOMA GROWTH FROM THE VIEWPOINT OF MOLECULAR BIOLOGY_ CRIMSON PUBLISHERS


An Evaluation on Oral Vestibular Schwannoma Growth from the Viewpoint of Molecular Biology by Bing H Tang* in Cohesive Journal of Microbiology & Infectious Disease: Crimson Publishers_ Infectious disease Journals


This author has known Medical Hypotheses several years ago, unfortunately did not know that it is available online lately, until recently when he read Prof. Gong Peng’s important hypotheses. Has been honored to receive the invitation from Prof. Ping Gong [an oral surgeon] for our potential collaboration. In the meantime, please correct some possible errors in this author’ writing as presented below, and the possibility of applying his own concepts into the important future research.

This author read with great interest the article written by Baser. Predictors of vestibular schwannoma growth in patients with neurofibromatosis. Their research expresses result of two longitudinal studies of growth rates of vestibular schwannomas (VSs) in patients with neurofibromatosis Type 2 (NF2) differs from each other as to whether VS growth rates decrease or increase when ever patients getting older. The authors undertook their study to assess the relationship between VS growth rates and patient age and type of constitutional NF2mutation; they also examined the in VS growth rates among multiple patients in families with NF2. Methods by using Gadolinium-enhanced magnetic resonance images obtained in 18 patients with inherited NF2 from 11 unrelated families whom were retrospectively analyzed. The patients had been observed for a period with a median of 4 years. Volumes of the VSs were checked using a two component box model (intrameatal and extrameatal parts checked respectively)


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Friday, May 10, 2019

Are There Vaccines Against Violence?-Crimson Publishers


Are There Vaccines Against Violence? by Joao Paulo Martins do Carmo in Cohesive Journal of Microbiology& Infectious Disease: Crimson Publishers_ Infectious disease Journals




Considering the transdisciplinar character of this Cohesive Journal of Microbiology and Infectious Disease, I would like to approach the violence and security question from the point of view of the Public Health, comparing them, shortly, to the cancer development (carcinogenesis), once cancer and violence are both Public Health problems. I also would like to approach it according to the holistic and ecologic theory of Fritj of Capra from his seminal masterpieces “The Web of Life” and “Turning Point”.


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Wednesday, May 1, 2019

Review on (Yelow Pigment) Citrinin C CRIMSON PUBLISHERS Wings to the Research


Review on (Yelow Pigment) Citrinin C CRIMSON PUBLISHERS Wings to the Research

Vasanthabharathi V* and Jayalakshmi S
Faculty of Marine Sciences, S India
*Corresponding author: Vasanthabharathi V, Faculty of Marine Sciences, Parangipettai, Tamil Nadu, India
Submission: October 10, 2018; Published: November 26, 2018

Introduction
The genus Penicillium contains approximately 100 toxigenic species, and the range of mycotoxin classes produced is much broader than that of any other genus [1]. listed 27 mycotoxins produced by 32 species which possessed varying range of toxicity. Penicillium toxins can be placed in two broad groups based on effect as renal and neurotoxins [2]. Mycotoxins are secondary metabolites produced by certain filamentous fungi. The toxic response due to fungal growth in food is called as mycotoxicosis. Aflatoxins are produced mainly by A. flavus, A. parasiticus and A. nominus. Aspergillus, Fusarium and Penicillium [3] and when ingested through food they intoxicate higher vertebrates including human. Monascus pigments, which are produced by various species of Monascus, often have been used as a natural colourant and as traditional natural food additives, especially in Southern China, Japan and Southeastern Asia. The limitation of wide using Monascus pigment is attributed to one of its secondary metabolites named citrinin.s

https://www.researchgate.net/publication/329584351_Review_on_Yelow_Pigment_Citrinin_C_CRIMSON_PUBLISHERS_Wings_to_the_Research

Home Remedy for Malnourished Kids Works Wonders Case Report-Crimson Publishers


Home Remedy for Malnourished Kids Works Wonders Case Report-Crimson Publishers
Ashish Satav*
Head Administration, India
*Corresponding author:
 Ashish Satav, Head Administration, India, Tel: 91-9423118877; Email:
Submission:
 July 13, 2018;
 Published:
 September 10, 2018


Many severely malnourished (grade IV) children continue todie every year in Melghat because their parents are unwilling tohospitalize them. Myths and mis-beliefs too hold them back fromapproaching the hospital. However, doctors from MAHAN Trustworking in the tribal belt have proved that these children can bealso saved through home or community based treatment, bothmedical and dietary. Successful treatment of Lakshmi SukhramJamunkar, a 23-month-old girl whose condition was worse than agrade IV child, is a shining example. She was treated at her homeunder the supervision of an illiterate but well-trained village healthworker, Meerabai. Lakshmi‘s case shows that community level
treatment can work wonders provided it is taken up scientiically
by trained personnel.