The Evaluation of the Speed-Oligo® Mycobacteria Assay for Identification of Mycobacterium spp. from Smear Positive and Negative Sputum Samples: Crimson Publishers

The Evaluation of the Speed-Oligo® Mycobacteria Assay for Identification of Mycobacterium spp. from Smear Positive and Negative Sputum Samples by Gülnur Tarhan in Cohesive Journal of Microbiology & Infectious Disease

Speed-oligo® Mycobacteria is an oligochromatographic test for the qualitative detection of Mycobacterium genus and the species of M. tuberculosis complex, directly in clinical samples. It is based on polymerase chain reaction targeting 16S rRNA and 16S-23S rRNA regions and double-reverse hybridization on a dipstick using probes bound to colloidal gold and to the membrane. In this study, we evaluated 25 type reference strains of mycobacteria (18 non-tuberculosis mycobacteria, M. tuberculosis H37Rv and M. tuberculosis H37Ra), 60 sputum samples (40 smear positive, 20 smear negative) collected from patient with suspected TB. All results were compared with microscopy, Löwenstein Jensen culture and Inno-Lipa (GenoType Mycobacterium CM/AS; Hain Lifescience, Germany). All smear positive sputum samples were positive with microscopy, culture and Speed-oligo® Mycobacteria. Of 20 smear negative sputum samples, 7 were culture positive. Of 7 culture positive samples, 3 were positive with microscopy and Speedoligo ® Mycobacteria. It is not effective to identify for M. intermedium, M. kansasii and M. xenopi.

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Molecular Dıagnostic Tests Used in the Dıagnosis of Tuberculosis:Crimson Publishers

Molecular Dıagnostic Tests Used in the Dıagnosis of Tuberculosis: Crimson publishers_Cohesive Journal of Microbiology &Infectious Disease:  Infectious DiseaseOpen Access Journal: Crimson Publishers

The definitive diagnosis of tuberculosis (TB) is dependent on the isolation, identification and drug susceptibility testing of the causal agent Mycobacterium tuberculosis by cultivation. Smear microscopy has poor sensitivity and culture is slow to yield results. The resurgence of tuberculosis worldwide has been accompanied by an increase in the incidence of multidrug-resistant (MDR) tuberculosis on all continents. At the same time, a number of other nontuberculous mycobacterial (NTM) species are emerging as causes of disease. A quick and correct diagnosis of symptomatic tuberculosis is critical for the control of this serious disease. The nucleic acid amplification techniques (NAATs) and other molecular biology methods (i .e. DNA hybridization, DNA sequencing, etc.) are essential in today’s laboratory practices for detection and characterization of mycobacteria.

https://crimsonpublishers.com/cjmi/fulltext/CJMI.000532.php

For more open access journals in Crimson Publishers,
please click on link: https://crimsonpublishers.com/
For more articles in Infectious Disease Open Access Journals,
Please click on link: https://crimsonpublishers.com/cjmi/

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