Epidemiology of Onychomycosis in Pernambuco, Northeastern of Brazil: Results of a Laboratory-Based Survey_Crimson Publishers

Epidemiology of Onychomycosis in Pernambuco, Northeastern of Brazil: Results of a Laboratory-Based Survey by Patrícia Cristina Rodrigues in Cohesive Journal of Microbiology & Infectious Disease

https://crimsonpublishers.com/cjmi/fulltext/CJMI.000541.php

Onychomycosis is chronic ungual disease that affects people worldwide and whose prevalence has been increasing in recent decades. Among the etiological agents are yeasts, dermatophytes and non-dermatophytid filamentous fungi (NDFF). The symptoms and severity can vary widely according to the anatomic site, condition of the individual and geographic location. Laboratory identification of the fungal etiological agents is necessary due to the wide variety of these agents and the varied responses to the existing drugs. This study aimed to diagnose and characterize epidemiologically onychomycosis, besides of to determine the susceptibility profile against evaluable antifungals drugs. Patients of a Dermatological Public Service of reference at Recife city, Northeast of the Brazil, which were suffering from suggestive lesions were evaluated between August 2016 and July 2017. Mycological diagnosis was carried out by direct microscopic examination of clinical samples clarified with 20% potassium hydrochloride (KOH) and by culture in Sabouraud dextrose agar medium supplemented with 2% (v/v) chloramphenicol. After incubation for 15 days at 35-37 °C, the macro and microscopic aspects of the colonies were analyzed for specie identification. The yeasts were identified by proteomic analysis with MALDI-TOF MS. In vitro antifungal susceptibility testing was carried out according CLSI method. Signs and symptoms suggestive of onychomycosis were presented in 196 patients, of which were obtained 224 nail samples. In the mycological tests, 

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