Shrimp industry is one of the important sectors in the kingdom of Saudi
Arabia and it has shown steep production and economic values. WSSV is a
major pathogen which causing 100% mortality and economic losses in world
wide. In KSA two most important species such as
Litopenaeus vannamei
and
Fenneropenaeus indicus were cultured widely. Due to the sheer
expansion of shrimp culture industry, WSSV occurrences was found in F.
indicus
and followed by WSSV outbreak, SPF L. vannamei was imported and cultured
in KSA with stringent biosecurity measures implied by ministry of
water,
environment and agriculture. The present study was aims to identify the
presence of WSSV in L. vannamei in selected area of KSA applying
molecular
method. Samples were collected from Jazan region it was fixed in 95%
ethanol and stored in -80°C for the analysis. The collected samples were
subjected
to nucleic acid (
DNA) extraction, followed by conventional and real time
PCR. The obtained positive PCR products were purified, and it was
sequenced.
The sequences analyzed to see the similarities in NCBI blast.
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